Collaborations are most welcome. Please send your proposals to Dr. George J. Delinasios.
The following research projects are in progress at the IIAR in cooperation with other Cancer Research Centers.
A. Interactions between stromal fibroblasts and cancer cells in vitro
B. Mechanisms of development of resistance to cisplatin in vitro.
Brief Description of the Projects.
A. INTERACTIONS BETWEEN STROMAL FIBROBLASTS AND CANCER CELLS IN VITRO.
Considering recent advances and the current interest in the role of the tumor microenvironment
on cancer development and progression, this project is a continuation of earlier work:
- Delinassios JG, Hoffman RM. The cancer-inhibitory effects of proliferating tumor-residing fibroblasts. Biochim Biophys Acta Rev Cancer. 2021 Dec 22;1877(1):188673. doi: 10.1016/j.bbcan.2021.188673. Epub ahead of print. PMID: 34953931.
PDF - Lamprecht S, Sigal-Batikoff I, Shany S, Abu-Freha N, Ling E, Delinasios GJ, Moyal-Atias K, Delinasios JG, Fich A. Teaming Up for Trouble: Cancer Cells, Transforming Growth Factor-β1 Signaling and the Epigenetic Corruption of Stromal Naïve Fibroblasts. Cancers (Basel). 2018 Feb 27;10(3):61. doi: 10.3390/cancers10030061. PMID: 29495500; PMCID: PMC5876636.
PDF - Delinasios JG, Angeli F, Koumakis G, Kumar S, Kang WH, Sica G, Iacopino F, Lama G, Lamprecht S, Sigal-Batikoff I, Tsangaris GT, Farfarelos CD, Farfarelos MC, Vairaktaris E, Vassiliou S, Delinasios GJ. Proliferating fibroblasts and HeLa cells co-cultured in vitro reciprocally influence growth patterns, protein expression, chromatin features and cell survival. Anticancer Res. 2015 Apr;35(4):1881-916. PMID: 25862842.
Summary | PDF - Delinassios JG. Fibroblasts against cancer cells in vitro. Anticancer Res. 1987 Sep-Oct;7(5B):1005-10. PMID: 3324932.
Summary | PDF - Angeli F, Koumakis G, Chen MC, Kumar S, Delinassios JG. Role of stromal fibroblasts in cancer: promoting or impeding?Tumour Biol. 2009;30(3):109-20. doi: 10.1159/000218708. Epub 2009 May 14. PMID: 19440007.
Summary | PDF - Delinassios JG. Cytocidal effects of human fibroblasts on HeLa cells in vitro. Biol Cell. 1987;59(1):69-77. doi: 10.1111/j.1768-322x.1987.tb00517.x. PMID: 2955837.
Summary | PDF - Delinassios JG, Kottaridis SD. Interactions between human fibroblasts and HeLa cells in vitro.Biol Cell. 1984;50(1):9-16. doi: 10.1111/j.1768-322x.1984.tb00250.x. PMID: 6234042.
Summary | PDF - Delinassios JG, Kottaridis SD, Garas J. Uncontrolled growth of tumour stromal fibroblasts in vitro.Exp Cell Biol. 1983;51(4):201-9. doi: 10.1159/000163192. PMID: 6873436.
Summary | PDF - Delinassios J, G: Prolonged in vitro Maintenance of Human Diploid Fibroblasts in a New Tissue Culture Medium (PRC-1). Pathobiology 1983;51:315-321. doi: 10.1159/000163210
Summary | PDF - Delinassios J, G, Kottaridis S, D: Interactions between Human Lymphoblastoid Cells and Human Fibroblast Feeder Layers in vitro.Pathobiology 1981;49:177-184. doi: 10.1159/000163821
PDF - Delinassios JG, Pandis NB, Margaronis M, Kottaridis SD. Double minutes in fibroblast-like cells isolated from human tumors. 1981 Jun;37(6):569-70. doi: 10.1007/BF01990054. PMID: 7262278.
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B. MECHANISMS OF DEVELOPMENT OF RESISTANCE TO CISPLATIN IN VITRO.
Study of the initial stages of development of resistance to cisplatin in vitro, focusing on specific
chromosomal abnormalities: existence of HSRs, AgNOR, and SCEs.
This project is a continuation of earlier work:
- Delinassios JG, Hoffman MR. , Koumakis G, Palitskaris D, Poulatsidou K-N, Delinasios GJ. Sub-toxic cisplatin concentrations induce extensive chromosomal, nuclear and nucleolar abnormalities associated with high malignancy before acquired resistance develops: Implications for clinical caution. , PLoS ONE 19(12):e0311976. https:/doi.org/10.1371/journal.pone.0311976
PDF - Delinassios J.G, Talieri M.J. Increased dihydrofolate reductase, double minutes and increased nucleolar activation in methotrexate-resistant HeLa cells. Experientia 39: 1394-1395, 1983
Summary | PDF - Delinassios J.G, Talieri M.J. Cytogenetical changes during early stages of development of methotrexate resistance in Hela cells. Medical Oncology & Tumor Pharmacotherapy 2:33-38, 1985
Summary | PDF - Delinassios J.G, Talieri M.J. Chromosomal aberrations in early stages of development of methotrexate resistance in Hela cells. Experimental Cell Biology 55:69-73, 1987
Summary | PDF