Collaborations are most welcome. Please send your proposals to Dr. John G. Delinasios or Dr. George J. Delinasios.
The following research projects are in progress at the IIAR in cooperation with other Cancer Research Centers.
- Invasion and metastasis: In vitro and in vivo models.
- In vitro and in vivo preclinical evaluation of new anticancer agents.
- Mechanisms of development of resistance to cisplatin in vitro.
- The biochemical pathways of pyrimidine and purine synthesis as targets of combination chemotherapy with new anticancer agents (in the process of organization).
Brief Description of the Projects.
A. INVASION AND METASTASIS: IN VITRO AND IN VIVO MODELS.
Study of the initial development stages of the invasive properties of tumor cells; Heterogeneity of the invasive cells; Interactions between stromal fibroblasts and tumor cells; In vitro and in vivo evaluation of the new antimetastatic agents; In vitro antimetastatic drug assay.
INTERACTIONS BETWEEN STROMAL FIBROBLASTS AND TUMOR CELLS
This is one of the main projects of the IIAR.
Considering recent advances and the current interest in the role of tumor microenvironment on cancer development and progression, this project is a continuation of earlier work:
- CANCERS (Basel) 10(3).pii. E61, 2018 (DOI: 10.3390/cancers10030061)
- ANTICANCER RESEARCH 35: 1881-1916, 2015
- ANTICANCER RESEARCH 7: 1005-1010, 1987
- Tumor Biology 2009:30:109-120 (DOI: 10.1159/000218708)
- Biology of the Cell 59:69-78, 1987
- Biology of the Cell 50:9-16, 1984
- Experimental Cell Biology 51:201-209, 1983
- Experimental Cell Biology 51:315-321, 1983
- Experientia 37:369-370, 1981
B. IN VITRO AND IN VIVO PRECLINICAL EVALUATION OF NEW ANTICANCER AGENTS.
Phenothiazine derivatives; Compounds of Gallium, Ruthenium and Vanadium; New 5- substituted pyrimidine derivatives; Cell cycle specific targets; New methodologies involving single cell assays, chromosomal aberrations, induction of apoptosis, specific enzyme activity on single cells, in situ hybridization; Methioninase as a tumor selective therapeutic modulator.
C. MECHANISMS OF DEVELOPMENT OF RESISTANCE TO CISPLATIN IN VITRO.
Study of the initial stages of development of resistance to cisplatin in vitro, focusing on specific chromosomal abnormalities, existence of HSR, AgNOR, morphology and SCEs.
This project ia a continuation of earlier work:
- Experientia 39: 1394-1395, 1983
- Medical Oncology & Tumor Pharmacotherapy 2:33-38, 1985
- Experimental Cell Biology 55:69-73, 1987
D. THE BIOCHEMICAL PATHWAYS OF PYRIMIDINE AND PURINE SYNTHESIS AS TARGETS OF COMBINATION CHEMOTHERAPY WITH NEW ANTICANCER AGENTS
Investigation of the possibilities of blocking simultaneously several biochemical pathways leading to the synthesis of pyrimidine and purine nucleotides. Combinations of recently developed compounds, which show remarkable activity against enzymes involved in the de novo synthesis of pyrimidine nucleotides will be used for this purpose. A series of established and primary human cancer cell lines will be used in the in vitro chemosensitivity assays for these combinations. The results are expected to identify new targeting methodologies against cancer, which may then be directly applicable in the chemotherapeutic treatment of cancer patients.